Title : To study the possibility of ape1/ref-1 as a novel biomarker in the detection of coronary artery disease
Abstract:
Coronary Artery Disease, is the leading cause of death globally resulting in over 7 million deaths. Apurinic/apyrimidinic endonuclease 1/redox effector factor 1 (APE1/Ref-1) is a multifunctional protein that is mainly located in the nucleus. APE1 is involved in the base excision repair pathway, and Ref-1 acts as a reductive activator of many transcription factors in controlling different cellular process such as apoptosis, inflammation, proliferation, angiogenesis, and is involved in survival pathways. The study aimed to investigate the possibility of APE1/Ref-1 as a novel biomarker in the detection of Coronary Artery Disease in Indian population, and to correlate the levels of APE1/Ref-1, NT-proBNP and Lipid Profile in CAD patients. The study included 100 subjects divided into two groups of cases and controls. The Case group included Fifty patients diagnosed of Coronary Artery Disease based on Clinical findings with Electrocardiography, Biochemical Markers, Echocardiography and Angiogram(wherever possible) attending the hospital OPD. Fifty age and gender matched healthy individuals constituted the control group. Quantitative variables are expressed as mean±sd and compared between groups using Unpaired t-test/Mann-Whitney test. Qualitative variables are expressed as frequencies/percentages and compared using Chi-square/Fisher’s Exact test. The mean APE 1 in case group was 3.77 ±1.03 whereas in control group was 1.20±0.7, with p-value of <0.001. A p value of <0.05 implies statistically significant difference in mean APE1 between case and control group. Our findings suggest that APE1/Ref-1 has a positive correlation with in patients of CAD. The study also suggest a positive correlation between abnormal Lipid Profile, raised NT-proBNP, and APE1/Ref-1, which further supports that APE1/Ref-1 can in-turn become a positive biomarker in diagnosis and prognosis of CAD.
What will audience learn from your presentation?
- Knowledge of APE1/Ref-1, a multifunctional protein
- Open horizon for more in-depth research for development of a new novel bio-marker for CAD
- Knowing the correlation of APE1/Ref-1, NT-proBNP and Lipid Profile in Coronary Artery Disease patients
